P-345 SOX-2 promotes epithelial-to-mesenchymal transition by modulating SLUG expression in esophageal squamous cells

Esophageal cancer is the sixth leading cause of cancer-related deaths worldwide, and majority these cancers, arises from squamous cells. Despite multiple therapeutic strategies, five year survival rate esophageal cell carcinoma (ESCC) ranges between 15%-20%. Epithelial-mesenchymal transition (EMT) one key events in ESCC, which associated with recurrence, metastasis low rate. Cancer stem cells (CSCs) are a heterogeneous population within that can differentiate into various types often linked to relapse. Earlier studies have reported that, exhibiting EMT acquire cell-like characteristic express markers EMT. However, correlation stemness still unclear ESCC. This study was done evaluate if marker SOX-2 promote by modulating expression SLUG Squamous The ESCC (Eca-109) were used for transfection coding DNA sequence, inserted pCMV vector. migration invasion evaluated using transwell chamber. marker, SLUG, overexpressing SOX-2, measured RT-PCR Western blot. explore, involved mediated regulation Eca-109/pCMV-SOX2 showed higher levels SOX2 mRNA, when compared Eca-109/pCMV, as demonstrated RT-PCR. transmigration assay revealed Eca-109/pCMV – cells, had increased invasive migratory potential after 24 hours incubation. Our also mRNA protein, positively correlated overexpression. demonstrates over promotes invasion, thus emphasizing usefulness target treatment